Table 1. Putative homologs of autophagy-related proteins have been identified in the genome of P. berghei.
| Autophagy-related proteins | P. berghei homologs | Expectation value | % pairwise identity with yeast | Reciprocal BLAST hit No. | |
|---|---|---|---|---|---|
| Expansion and completion |
Atg8 |
PBANKA_050401 |
2.2e-27 |
45 |
1 |
|
Atg4 |
PBANKA_102540 |
3.5e-7 |
11 |
1 |
|
|
Atg3 |
PBANKA_041570 |
1.4e-38 |
29 |
1 |
|
|
Atg7 |
PBANKA_092220 |
3.3e-50 |
21 |
1 |
|
|
Atg12 |
PBANKA_133320 |
1.2e-7 |
15 |
9 |
|
| Atg10 |
PBANKA_101190 |
0.14 |
9 |
1 |
|
| Atg5 |
PBANKA_101430 |
0.03 |
11 |
- |
|
| Atg16 | PBANKA_082930 | 0.0001 | 24 | - |
Apart from the Atg4 protein (underlined) all members of the Atg8 ubiquitin-like conjugation pathway are highly conserved in P. berghei (bold). Proteins of the Atg12 ubiquitin-like conjugation system either show a low conservation (underlined) or are not present at all. S. cerevisiae Atg protein sequences were used to search for P. berghei homologs using the BLAST function of PlasmoDB (expectation values of this search are presented). Percentage of pairwise identity with yeast was acquired via Needleman-Wunsch alignment. The hit number of reciprocal BLAST search against UniProtKB/Swiss-Prot(swissprot) database of the organism S. cerevisiae is indicated.