Figure 1. LC3-I to LC3-II turnover in oxidatively stressed TM cells. (A) Protein expression levels of LC3-I, LC3-II, and SQSTM1 in TM cells grown under physiological 5% O₂ or oxidative 40% O₂ conditions for two weeks in the presence or absence of Leup (10 μM, twice per week), evaluated by WB analysis. TUBB/β-tubulin was used as loading control. (B) Expression levels of LC3-I and LC3-II in TM cells grown under 5% O₂ or 40% O₂ conditions for two weeks followed by a three h treatment with CQ (30 nM). Blots are representative from three independent experiments.