Figure 3. Monitoring autophagy flux in TM cells using AdtfLC3. Porcine TM cells were transduced with AdtfLC3 (m.o.i = 10 pfu/cell). At 3 d.p.i., cells were treated for 24 h with rapamycin (Rap, 1 μM) or BafA1 (10 nM). Confocal images were taken, and red and yellow puncta per cell counted. Data are means ± SD of three independent experiments, ten cells/experiment, n = 30, *** p < 0.0001, **p < 0.001.