Figure 8. Autophagy inhibition in oxidatively stress TM cells. (A) Protein expression levels of LC3-I and LC3-II in TM cells grown under physiological 5% O2 or oxidative 40% O2 conditions for two weeks in the presence of 3-MA (0 mM, 0.5 mM, 1 mM added twice per week), evaluated by WB analysis. TUBB was used as loading control. Blots are representative from three independent experiments. (B) Effects of 3-MA in lipofuscin content, lysosomal mass, and SA-GLB1 in TM cells grown at 5% O2 and 40% O2. Graphs represent the percentage of change compared with the 5% O2 condition. Data are the means ± SD, n = 3, **p < 0.001, ***p < 0.0001.