Table 1. List of primary antibodies used.
| Target protein | Company | Catalog number | Dilution |
|---|---|---|---|
| LC3A/B |
Abcam |
ab58610 |
1:500 |
| LC3B |
Cell Signaling |
3868S |
1:1000 |
| SQSTM1 |
Sigma-Aldrich |
P0067 |
1:1000 |
| TUBB |
Sigma-Aldrich |
T5293 |
1:1000 |
| ATG5 |
Cell Signaling |
8540P |
1:1000 |
| BECN1 |
Cell Signaling |
3495P |
1:1000 |
| ATG7 |
Cell Signaling |
2631P |
1:1000 |
| ATG12 |
Cell Signaling |
4180P |
1:1000 |
| RAB7A |
Abcam |
ab50533 |
1:2000 |
| LAMP1 |
Abcam |
ab24170 |
1:1000 |
| CTSB |
Abcam |
ab58802 |
1:1000 |
| CTSD |
Santa Cruz Biotechnology |
SC-6494 |
1:500 |
| M6PR |
Hybridoma Bank |
22d4 |
1:1000 |
| Flag |
Sigma-Aldrich |
F1804 |
1:1000 |
| LMNA |
Santa Cruz Biotechnology |
SC-6215 |
1:500 |
| RPS6KB |
Santa Cruz Biotechnology |
SC-230 |
1:1000 |
| p-RPS6KB | Santa Cruz Biotechnology | SC-7984-R | 1:1000 |