Figure 4. Derivatives 15, 18 and 27 inhibit omegasome formation. (A) GFP-ZFYVE NRK cells were unstarved or starved for 2 h with or without 10 mM 3-MA, 1 mM 15, 1 mM 18 or 0.1 mM 27, and imaged by confocal microscopy. Scale bars: 10 μm. (B) Cells from (A) were quantified for the number of omegasomes. At least 30 cells were counted. Error bars, s.d.; ***p = 0.0001 compared with starved cells by Student’s t-test.