Figure 5. Derivatives 15, 18 and 27 do not inhibit AKT phosphorylation. (A) NRK cells were incubated with 2 μM wortmannin, 10 mM 3-MA, 0.1 mM 27, 1 mM 15 or 1 mM 18 for 4 h and total cell extracts were immunoblotted with antibodies against AKT and phospho-AKT. (B) NRK cells were incubated with 10 mM 3-MA, 0.1 mM 27, 1 mM 15 or 1 mM 18 for the indicated time and total cell extracts were immunoblotted with antibodies against AKT and phospho-308 AKT. (C) Leupeptin (10 μg/ml)-treated NRK cells were incubated with or without 10 mM 3-MA, 1 mM 18, 1 mM 15 or 0.1 mM 27 for 4 h, and the processing of LC3 was measured by western blot using an anti-LC3 antibody.