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. Author manuscript; available in PMC: 2013 Apr 17.
Published in final edited form as: Circ Res. 2012 Aug 14;111(9):1166–1175. doi: 10.1161/CIRCRESAHA.112.275743

Figure 7. STIM1 downregulation attenuates the rise in [Ca2+]cyt due to Ca2+ release/leakage from the ER during CPA treatment and decreases [Ca2+]ER in coronary ECs.

Figure 7

A, STIM1 siRNA transfection in HCECs downregulates STIM1 protein expression determined by Western blot. Values are mean±SEM (n=2). *P<0.05 versus control siRNA. B, Summarized data of the rise in [Ca2+]cyt due to Ca2+ release/leakage from the ER (1st ΔF/F0 and 1st area under the curve [AUC]) and SOCE (2nd ΔF/F0 and 2nd AUC). Control siRNA (open bars), n=25 cells; STIM1 siRNA (solid bars), n=27 cells. Data are mean±SEM. *P<0.05 versus control siRNA. C, Summarized data of resting level of [Ca2+]ER, ΔPeak, and the AUC (the change in [Ca2+]ER after CPA treatment). Control siRNA (open bars), n=24; STIM1 siRNA (solid bars), n=30. Data are mean±SEM. *P<0.05 versus control siRNA.