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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Bioorg Med Chem. 2013 Mar 7;21(9):2503–2510. doi: 10.1016/j.bmc.2013.02.042

Figure 2. Effects of compounds 3-7 on SK1 activity.

Figure 2

Substrates were 3 μM sphingosine (which corresponds to the Km of SK1)7 and 250 μM ATP (n = 3, results expressed as mean inhibition +/− S.E.). Compounds 2-7 were not substrates for SK1 and were each used at 50 μM. The SK1-selective inhibitor BML-25835 (50 μM) inhibited SK1 activity by 74.5 +/− 3.3 % (n = 3). Sph denotes sphingosine. The control is set at 100% and represents the SK1 activity against sphingosine alone.