Figure 1.

Autocorrelation curves of RCC1-EGFP on chromatin and in the cytoplasm of HeLa cells at different stages in the cell cycle. Representative measured in the nucleus of an interphase HeLa cell (A), the cytoplasm of an interphase HeLa cell (B), on chromatin in a HeLa cell in metaphase (C), and in the cytoplasm of a HeLa cell in metaphase (D). (Curves in panels A and C were fit with the binding-diffusion model; curves in panels B and D were fit with a model for free diffusion.) (Upper panel of each graph) Fit residuals. (Insets) Confocal images of RCC1-EGFP expressing HeLa cells, with measurement positions indicated (open crosses). Scale bars: 5 μm.