Figure 3.

Equilibration of RCC1 between chromatin and cytoplasm in mitotic cells. (A and B) A HeLa cell expressing RCC1-EGFP (green) and Tubulin-mCherry (red) was arrested in metaphase with 100 ng/mL nocodazole and fluorescence was imaged over a period of over 1 h. Scale bar: 5 μm. Average cytoplasmic and chromatin fluorescence intensities of RCC1-EGFP were used to calculate the fraction of unbound RCC1 at each time point (B). RCC1-paGFP was photoactivated on mitotic chromatin and the cytoplasmic fluorescence intensity of photoactivated RCC1-paGFP followed in time (C).