Table 1.
Diffusion constants of EGFP, RCC1-EGFP, and NT-EGFP measured in different compartments of HeLa cells
| Protein | Compartment | D/μm2 s−1 | N |
|---|---|---|---|
| EGFP | Nucleus | 37.79 ± 4.37 | 15 |
| Interphase cytoplasm | 35.12 ± 6.31 | 15 | |
| Mitotic chromatin | 26.64 ± 4.36 | 14 | |
| Mitotic cytoplasm | 35.95 ± 6.94 | 14 | |
| RCC1-EGFP | Interphase cytoplasm | 27.11 ± 5.35 | 15 |
| Mitotic cytoplasm | 11.05 ± 1.66 | 15 | |
| NT-EGFP | Nucleus | 16.8 ± 4.06 | 15 |
| Mitotic chromatin | 12.9 ± 5.32 | 10 | |
| Mitotic cytoplasm | 17.94 ± 2.83 | 10 |
Diffusion constants were determined by fitting a model for free diffusion to the autocorrelation data. NT-EGFP refers to a fusion protein comprising the first 27 amino acids of RCC1γ fused to EGFP. Values given are mean ± SD calculated from measurements in N different cells.