Time-averaged mean-square displacement distributions for all experiments. Snapshots of the distribution for the three experimental conditions at four different lag times Δ. In the case of buffer, 28 trajectories are analyzed, in the untreated extract case, 40 are analyzed, and in the case of extract treated with nocodazole, 31 are analyzed. All figure axes mirror those in the bottom left, but are removed for clarity. The trend, in the cases of Buffer and Extract + noc (nocodazole), is independent of lag time whereas the case of Extract shows a shifting peak with increasing lag time. To see this trend more clearly, compare this figure with Fig. 4, which plots the distribution for many values of Δ.