Table 2. The effects of miR-203 on BaF3-BCR/ABLT315I cell cycle progressiona.
Cell type | Length of culture (h) | % of cells in indicated phase | ||
G1 | G2 | S | ||
BaF3-BCR/ABLT315I | 0 | 52.83 | 12.08 | 35.09 |
BaF3-BCR/ABLT315I-NC | 52.94 | 13.90 | 33.16 | |
BaF3-BCR/ABLT315I-miR203 | 53.23 | 13.64 | 33.13 | |
BaF3-BCR/ABLT315I | 2 | 44.43 | 14.58 | 40.99 |
BaF3-BCR/ABLT315I-NC | 46.20 | 14.31 | 39.50 | |
BaF3-BCR/ABLT315I-miR203 | 47.81 | 14.01 | 38.18 | |
BaF3-BCR/ABLT315I | 6 | 37.57 | 21.62 | 40.82 |
BaF3-BCR/ABLT315I-NC | 37.44 | 18.29 | 44.27 | |
BaF3-BCR/ABLT315I-miR203 | 43.56 | 15.59 | 40.85 | |
BaF3-BCR/ABLT315I | 12 | 26.70 | 23.33 | 49.97 |
BaF3-BCR/ABLT315I-NC | 27.14 | 22.09 | 50.77 | |
BaF3-BCR/ABLT315I-miR203 | 40.20 | 20.31 | 39.50 | |
BaF3-BCR/ABLT315I | 24 | 19.15 | 3.90 | 76.96 |
BaF3-BCR/ABLT315I-NC | 22.32 | 2.08 | 75.60 | |
BaF3-BCR/ABLT315I-miR203 | 31.85 | 4.41 | 63.74 |
Cells were incubated for 48 h in serum-free medium to synchronize cells in the G1 phase at 0 h. After that, cells were incubated in the medium with 10% FCS and collected at 2 h, 6 h, 12 h, and 24 h. The percentages of cells in G1, G2, and S phases were evaluated by flow cytometry.