Fig. 3.

H₂O₂ production. H₂O₂ production in SSM (A) and IFM (B) was determined using the oxidation of the fluorogenic indicator amplex red in the presence of horseradish peroxidase. H₂O₂ production was initiated in mitochondria using glutamate-malate as substrates. Standard curves were obtained by adding known amounts of H₂O₂ to the assay medium in the presence of the substrates amplex red and horseradish peroxidase. Final values were calculated as pmol/mg of protein. Values are expressed as means ± SE. *P < 0.05 for diabetic vs. all groups.