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. 2013 Feb 6;304(7):R543–R552. doi: 10.1152/ajpregu.00567.2012

Table 1.

Forward and reverse primer sequences for quantitative real-time PCR

Primer sequence (5′→3′)
Gene Forward Reverse
18S GCCGCTAGAGGTGAAATTCTTG CATTCTTGGCAAATGCTTTCG
Leptin GTGCTGAGAGGACTTTGTGGGCC CCTTCGCTCCAGACTGTGGCAC
TNF-α CCCAGAAAAGCAAGCAACCA CCTCGGGCCAGTGTATGAGA
MCP-1 CTGTCTCAGCCAGATGCAGTTAA AGCCGACTCATTGGGATCAT
IL-6 AGAGACTTCCAGCCAGTTGC AGCCTCCGACTTGTGAAGTG
IL-10 CTGGCTCAGCACTGCTATGT GCAGTTATTGTCACCCCGGA
IL-18 ACAGCCAACGAATCCCAGAC ATAGGGTCACAGCCAGTCCT
IL1-RA GAGGCTGATCATCCCGTGAG GCCAGAGTGATCAGGCAGTT
Adiponectin CAAGGCCGTTCTCTTCACCT CCCCATACACTTGGAGCCAG
OB-Rb GCAGCTATGGTCTCACTTCTTTTG GGTTCCCTGGGTGCTCTGA
TNFR1 TTGTAGGGATTCAGCTCCTGTC CTCTTACAGGTGGCACGAAGTT
TNFR2 TGCAACAAGACTTCAGACACCGTG AGGCATGTATGCAGATGGTTCCAG
IL-6R AAGCAGGTCCAGCCACAATGTAG CCAACTGACTTTGAGCCAACGAG
AdipoR1 GCAGACAAGAGCAGGAGTGT ACTGTGGTGGCCTTGACAAA
F4/80 GCCATAGCCACCTTCCTGTT ATAGCGCAAGCTGTCTGGTT
CD11c CTGTCATCAGCAGCCACGA ACTGTCCACACCGTTTCTCC
FoxP3 CTCCAGTACAGCCGGACAC GGTTGGGCATCAGGTTCTTG
PPAR-γ TGCTGCAGGCCCTGGAACTC AGCACCTTGGCGAACAGCTGG
UCP-1 CCGGTGGATGTGGTAAAAAC CTCCAAGTCGCCTATGTGGT