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. 2013 Feb 13;304(8):R644–R650. doi: 10.1152/ajpregu.00418.2012

Fig. 2.

Fig. 2.

ATGL-skeletal muscle lipid droplet proteins (PLIN) interactions at rest and following stimulated contraction. A: PLIN2 protein content (arbitrary units) in ATGL immunoprecipitated samples at rest and following contraction. Representative Western blot for ATGL-PLIN2 protein interaction, shown as IP ATGL and Western blot for PLIN2. Lane 1, standard, lanes 2–4 rest samples, lane 5 blank, lanes 6–8, stimulated samples; lane 9, empty; lane 10, soleus whole homogenate used as a positive control. *Significant decrease in ATGL-PLIN2 association following stimulated contraction (P = 0.028). B: PLIN3 protein content (arbitrary units) in ATGL-immunoprecipitated samples at rest and following contraction. Representative Western blot for ATGL-PLIN3 protein interaction, shown as IP ATGL and Western blot for PLIN3. Lane 1, standard, lanes 2–4, rest samples, lane 5, blank (Note IgG band at ∼50 kDa); lanes 6–8, stimulated samples; lane 9, empty; lane 10, whole soleus homogenate. No significant difference in ATGL-PLIN3 association following stimulated contraction (P = 0.266). C: PLIN5 protein content (arbitrary units) in ATGL immunoprecipitated samples at rest and following contraction. Representative Western blot for ATGL-PLIN5 protein interaction, shown as IP ATGL and Western blot for PLIN5. Lane 1, standard; lanes 2–4, rest samples; lane 5, blank (absent antibody interference); lanes 6–8, stimulated samples; lane 9, soleus whole homogenate used as positive control. No significant difference in ATGL-PLIN5 association following stimulated contraction (P = 0.591).