Fig. 2.
Hypoxia increases p27 and decreases cyclin D2 (D2) in fetal hearts and H9c2 cells. Protein abundance of D2 and p27 was determined by Western blots in fetal rat hearts and H9c2 cells, treated with 20% O2 or 1% O2 for 48 and 24 h, respectively. β2-Microglobulin (B2M) was used as a loading control. Data are means ± SE. *P < 0.05, 1% O2 vs. 20% O2. n = 5 per group.