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. 2013 Feb 20;304(8):R613–R620. doi: 10.1152/ajpregu.00515.2012

Fig. 5.

Fig. 5.

Knockdown of TIMP-3 or TIMP-4 blocks hypoxic effect on cardiomyocyte proliferation in H9c2 cells. H9c2 cells were transfected with siRNA against TIMP-3 or TIMP-4, respectively, for 24 h followed by the treatment with 20% O2 or 1% O2 for 24 h. Cardiomyocytes are stained for α-sarcomeric actinin (green) and Ki-67 (red). Nuclei were visualized using Hoechst 33258 (blue). A: negative control of siRNA (NC) at 20% O2; B: NC at 1% O2; C: TIMP-3 siRNA at 20% O2; D: TIMP-3 siRNA at 1% O2; E: TIMP-4 siRNA at 20% O2; F: TIMP-4 siRNA at 1% O2. Data are means ± SE. *P < 0.05, 1% O2 vs. 20% O2; #P < 0.05, TIMP-3 siRNA vs. negative control of siRNA (NC). n = 6 per group.