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Analysis of nitric oxide/iNOS expression. Panel A: Fluorescence micrographs of neonatal rat cardiomyocytes loaded with DAF-2DA. Cells were treated with Phenylephrine (PE: 10 μM), IL-6 (50 ng/μl), LIF (50 ng/μl) and LPS (10 μM) for 24 h in serum-free medium. Control myocytes (C) were left untreated. Panel B: Western blot containing 60 μg of protein per lane from control and treated cardiomyocytes immunoblotted with anti-iNOS antibody.
