Table 1.

Percentage of RGCs and other retinal cells labeled with GCaMP3 following intravitreal injection

	AAV2-CAG	AAV2/1-SYN1	P Value
RGCs labeled, %	82.9 ± 9.2	75.4 ± 7.5	0.14
Labeled cells that were not RGCs, %	14.7 ± 7.6	17.6 ± 3.9	0.39
Labeled cell density, cells per square millimeter	1,813 ± 343	1,553 ± 367	0.24
No. counted cells	2,714	1,492

Percentages indicate means ± SD. Cells were counted in regions with dense genetically encoded calcium indicator GCaMP3 labeling. We counted 8 400- × 400-μm regions from 2 retinas transduced with AAV2-CAG-GCaMP3 and 5 400- × 400-μm regions from 2 retinas transduced with AAV2/1-SYN1-GCaMP3. Retinal ganglion cells (RGCs) were identified after retrograde loading with Alexa Fluor 594. P values imply no significant differences between the labeling profile of each vector.