Fig. 5.
Dose-dependent reduction of outward current by intracellular QX-314 in DRG neurons. A: the current-voltage relationship for current activated by 1 μM capsaicin was determined in DRG neurons after dialysis with various concentrations of QX-314 in the intracellular solution. Currents were normalized to the current at −80 mV. B: collected results for reduction in capsaicin-activated outward current by intracellular QX-314, plotted as the ratio of current at +80 mV to current at −80 mV (I+80/I−80). C: reduction of outward current following loading of QX-314 by exposure to external QX-314 (10 mM) and capsaicin (1 μM) for 6 min. Current-voltage curve was determined after washout of external QX-314. D: collected results in 11 DRG neurons for change in rectification produced by exposure to 10 mM QX-314 and 1 μM capsaicin for times ranging from 100 to 350 s.