Figure 2.

A montage of the frames of immunopositive kisspeptin neurons in the arcuate nucleus produced by the sequential imaging option of the Bioquant software that was used to count kisspeptin neurons in coronal hemisections of this nucleus. The motorized stage of the microscope was moved sequentially from frame 1 (bottom left) to frame 12 (top right), and the number of kisspeptin perikarya in each frame was counted under high power (×40) and recorded on a spread sheet. ARC, arcuate nucleus; ME, median eminence; 3V, third ventricle.