Immunogold labeling of hair bundles for parvalbumin-3. A–C: labeling of hair bundles from apical (A), middle (B), and basal (C) SHCs with parvalbumin-3 antibody. D–F: labeling of hair bundles from apical (D), middle (E), and basal (F) THCs with parvalbumin-3 antibody. Note for both cell types that there is an increase in label from apex to base but the SHCs are more heavily labeled at all locations than the THCs. Papilla locations: apex, d = 0.25; middle, d = 0.35; base, d = 0.70. P1 bird.