Figure 1. Female reproductive glands are essential for ovulation.
(A)–(C) DIC images of Oregon-R (A), Hr397154/Ly92 (B) and lz3/34 (C) mutant female lower reproductive tracts. Both spermathecae (yellow arrowheads) and parovaria (magenta arrows) are absent in the mutant animals. Bar graphs display the rate of egg laying (D and E), ovulation frequency (F and G), and copulation frequency (H and I) for the two mutant genotypes, and heterozygous controls. In all figures, the number of egg laying groups or mating pairs is shown in brackets. Error bars are SEM, or 95% confidence intervals. *p<0.01 (Fisher's exact test, or Student t-test).