Figure 3. Notch signaling and Hindsight are required to form reproductive gland secretory cells.
(A) The cell lineage underlying secretory cell development (Sun and Spradling, 2012). Notch signaling activity (green); Hnt expression (red). Ac: Apical cell; Bc: Basal cell; LEP: Lumen epithelial precursor; Sc: Secretory cell; SUP: Secretory unit precursor. (B)–(D) Notch activity (green) and Hnt (red) in spermathecae of adults (B), 26 hr pupae (APF) (C), and 48 hr APF (D). (D') shows the boxed region from (D). Yellow arrowhead: Epithelial cell. (E)–(G) Adult spermathecae from females expressing lz>Psn[DN] (E) or lz>hntRNAi (F–G) during gland development. lz (green) marks epithelial cells; Hnt (red) marks secretory cells.