Figure 5. Canonical protein secretion from glandular secretory cells is required for sperm storage but not for ovulation.
(A) Experimental scheme for testing adult secretory cell function using temperature sensitive shits or GAL80ts. (B) and (C) Dynamin (Shi) is required for sperm storage. Female reproductive tract of syt12Gal4 control (B) or syt12Gal4 driving shits expression (C) 6 hr after mating to protB-GFP males at 29°C. syt12Gal4 expression is restricted to secretory cells as showed by UAS-RFP (red). (B') and (C'): Higher magnification of boxed spermathecae; seminal receptacle contain sperm (white arrows). (D) Sperm content of spermathecae (three classes) is reduced in flies with indicated genotype (x axis) at 29°C. Bracket: Number analyzed. *p<0.01 (chi-square test). (E) and (F) Abnormal morphology of spermathecal sperm in shits females at 29°C (F) compared to control (E). Egg laying rate (G) and ovulation time (from Table 2) (H) in flies with the indicated genotypes (x axes). *p<0.05 (Students t-test or Fisher's exact test). (I) Secretory cells use distinct secretory pathways to control sperm storage and ovulation.
Figure 5—figure supplement 1. Membrane trafficking defects are observed in SCs when protein secretion is disrupted.
