Figure 6. Acute stress increases FGF2 expression in dorsal hippocampus.
(A) 3 hr of immobilization increased fgf2 mRNA expression over control in dorsal hippocampus. Other groups did not significantly differ from control. One-way ANOVA, p=0.05. **q = 3.54, p<0.01. (B) 40 mg/kg CORT increased fgf2 mRNA expression in the dorsal hippocampus 3 hr after CORT injection compared to 30 min after 0 mg/kg CORT injection. Other groups did not significantly differ from oil-injected controls. Two-way ANOVA, effect of CORT, p=0.03; effect of time, p<0.0001; interaction, p=0.0021. ***q = 4.34, p<0.001. (C) FGF2 protein levels in dorsal hippocampus increased with 3 hr of immobilization over control. Other groups did not significantly differ from control. One-way ANOVA, p>0.05. *q = 2.79, p<0.05. (D) FGF2 protein levels in dorsal hippocampus increased 3 hr after 40 mg/kg CORT injection compared to 30 min after 0 mg/kg vehicle injection. Two-way ANOVA, effect of CORT, p=0.01; effect of time, p=0.03. *q = 3.18, p<0.05. (E) 3 hr of immobilization decreased bdnf exon IV expression over control in dorsal hippocampus. Other groups did not significantly differ from controls. One-way ANOVA, p=0.0007. *q = 3.05, p<0.05. (F) There was an overall significant decrease in bdnf exon IV mRNA expression with increasing CORT dose in dorsal hippocampus. Two-way ANOVA, effect of CORT, *p=0.02. (G) BDNF protein levels in dorsal hippocampus did not change with immobilization, novel environment or shock compared to control. (H) BDNF protein levels did not change compared to 0 mg/kg vehicle with increasing CORT dose. (I) Representative western bands of FGF2, BDNF and ACTIN from the 3 hr time point in dorsal hippocampus. (J) 3 hr of immobilization increased fgf2 mRNA expression over control in ventral hippocampus. Other groups did not significantly differ from controls. One-way ANOVA, p=0.03. *q = 2.87, p<0.05. (K) 40 mg/kg CORT increased fgf2 mRNA expression in the ventral hippocampus 3 hr after CORT injection compared to 30 min after 0 mg/kg CORT injection. Other groups did not significantly differ from oil-injected controls. Two-way ANOVA, effect of time, p=0.002. **q = 3.34, p<0.01. (L) Immobilization, novel environment and shock did not alter FGF2 protein levels in ventral hippocampus. (M) CORT did not alter FGF2 protein levels in ventral hippocampus 3 hr after injection. (N) Representative western bands of FGF2 and ACTIN from the 3 hr time point in ventral hippocampus. All values are average ± SEM.