Table 1.
List of viral constructs, serotypes, titers, and mouse lines used.
| Expression Cassette | Serotype | Viral particles/ hemisphere |
Mouse strain | Figure |
|---|---|---|---|---|
| CBA-tdTomato-2A-tTA(2S) | AAV8 | 4.0×109 | ICR | Figure 4 |
| 1.0×107 - 1.0×1010 | C57BL/6J | Figure 6 | ||
| 2.0×109 | C57BL/6J | Figure 7 | ||
| 8.0×108 | C57BL/6J | Figure 8 | ||
| 5.0×109 | TetO-LacZ/GFP reporter mice | Not shown | ||
| CBA-YFP-2A-tTA(2S) | AAV8 | 2.0×1010 | C57BL/6J for all | Figure 2 |
| 2.0×109 | Figure 5 | |||
| 4.0×107 - 2.0×1010 | Figure 6 | |||
| CBA-YFP-2A-tTA(2S) | AAV1 | 2.0×109 - 2.0×1010 | C57BL/6J for all | Figure 6 |
| 2.0×1010 | Figure 7 | |||
| 2.0×109 | Figure 8 | |||
| CBA-YFP-2A-YFP-2A-YFP | AAV8 | 4.0×108 | R26R Cre-reporter mice | Figure 9 |
| 1.0×108 | ICR | Figure 10 | ||
| 3.8×109 | ICR | Figure 11 | ||
| 1.0×108 | ICR | Figure 11 and Videos 1, 2 | ||
| CBA-YFP-2A-YFP-2A-YFP | AAV1 | 1.3×1010 | C57BL/6J | Figure 4 |
| 1.3×1010 | ICR | Figure 11 | ||
| EF1α-iCre-2A-tdTomato | AAV8 | 1.2×109 | Ai3 Cre-reporter mice | Figures 3 and 4 |
| 2.0×109 | R26R Cre-reporter mice | Figure 9 | ||
| EF1α-iCre-2A-tdTomato | AAV6 | 1.2×1010 | ICR | Figure 4 |