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. 2013 Apr 16;8(4):e61705. doi: 10.1371/journal.pone.0061705

Figure 6. Analysis of the transgenic Arabidopsis plants overexpressing UGT74D1 using the CaMV35S promoter.

Figure 6

(A) RT-PCR analyses of the steady-state level of UGT74D1 mRNA in transgenic plants (OEs) and wild type (WT). (B) The glycosyltransferase activities in the crude protein extracts of transgenic plants and wild type were measured following the procedure described under “Materials and Methods”. The specific enzyme activity was expressed as pmol of IBA glucosylated to form IBA-Glc by 1 mg of protein per second of reaction time at 37°C.