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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Mol Genet Metab. 2013 Feb 13;109(1):21–27. doi: 10.1016/j.ymgme.2013.02.002

Figure 2.

Figure 2

Figure 2

Immunofluorescent staining of wild type (A) and patient (B) fibroblasts. A. Wild type cells (WT). VLCAD antigen was visualized with green fluorescently tagged antibodies (top, middle panel) and mitochondrial cytochrome c oxidase (COX) was visualized with red fluorescently tagged antibodies (top, right panel). Nuclei were visualized with DAPI staining (blue; top, left panel). The merged image (bottom panel) shows colocalization of VLCAD and COX in mitochondria as yellow (white arrow). B. Patient fibroblasts. Each panel shows the merged only image of patients (PTs 1–6 and 8–11) with the corresponding Western blot results. The mutations identified in the cell line are shown at the top of each panel for convenience. Scale bar, 10.75 μm.