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. Author manuscript; available in PMC: 2014 Apr 11.
Published in final edited form as: Mol Cell. 2013 Feb 21;50(1):29–42. doi: 10.1016/j.molcel.2013.01.022

Figure 1. Rab5 plays a critical role in p110β-mediated autophagy.

Figure 1

β+/+ and β−/− MEFs were transfected with GFP-LC3 together with indicated expression constructs. (A) Western blots of cell lysates were probed with the indicated antibodies. Ponceau S staining shows equal protein loading. (B) Cells were imaged under a deconvolution microscope. Cells with more than 10 cytosolic puncta and diminished nuclear GFP were considered as autophagic. Data are averages of at least 4 blind countings with over 200 cells. Error bars: SD. * p<0.005. (C) Representative images of GFP-LC3 fluorescence in MEFs. Scale bar: 20 μm. (D) β+/+ and β−/− MEFs were transfected with vector (control), GFP-Rab5-WT, or GFP-Rab5-CA. 48 h later, cells were left untreated or treated with 50 nM bafilomycin A1 for 6 h. Relative levels of LC3-II against β-tubulin from three independent experiments are shown on the right. Data were normalized against that of β+/+. Error bars: SEM; * p<0.05; N.S., non-significant.