Fig. 6.

Eosinophil and neutrophil recruitment confirmed by flow cytometry. (a) Percentages of bronchoalveolar lavage (BAL) eosinophils and neutrophils at 1·5 and 16 h post-cockroach allergen (CRA) challenge, as determined by light microscopy. Identification of eosinophils (CD193⁺ Ly6G^low) and neutrophils (CD193^-Ly6G^high) was performed. Relative cell percentages at (b) 1·5 h and (c) 16 h post-CRA challenge. Flow cytometry confirms that the early BAL cell recruitment was composed primarily of eosinophils with almost no neutrophils at this time. By 16 h both neutrophils and eosinophils represent a significant percentage of BAL cell recruitment. Data for (a) are mean ± standard deviation of the mean for seven to eight mice from two separate experiments; ***P < 0·001 eosinophils versus neutrophils. Flow cytometry pictographs representative of four mice per time-point.