Figure 4.

PAR1 Inhibits PIF4-Mediated Transcriptional Activation.

(A) Quantitative RT–PCR analysis of the expression levels of PIL1, HFR1, and IAA29 in the Col-0, pifq, and PAR1OX plants. Seedlings were grown under red light for 5 d. Data were normalized to PP2A gene as internal control. Error bars indicate S.E. of three biological repeats.

(B) PAR1 inhibits PIF4-mediated PIL1 promoter activation. The reporter construct containing a 2-kb PIL1 promoter placed upstream of the luciferase reporter (PIL1p::luc) was co-transformed into protoplasts with the indicated effector vectors. Luc activity was normalized by renilla luciferase activity (internal control). Error bars indicate S.E. of three biological repeats.