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hnRNP A1 siRNA knockdown in MCF-10A and MCF-7. Following hnRNP A1 siRNA transfection, cells were harvested at various times (24–228 h) and hnRNP A1 protein levels evaluated (Western blot); graphs represent hnRNP A1 levels normalized to β-tubulin. Optimal knockdown of hnRNP A1 protein (∼90%) was achieved at 72 h for both cell lines (n = 4, each examined with two immunoblot analyses).
