Figure 6.

Total TERRA levels. Total RNA from unsynchronized cells was extracted and TERRA probed by dot blot and normalized to GAPDH (control). RNase treatment confirmed probe specificity for RNA. Comparisons examined for statistical analyses were: DNA-PKcs kinase inhibition (NU) vs. untreated (UT) and DMSO controls; or hnRNP A1 depletion (siRNA) vs. untreated (UT2) and mock controls. (A) Total levels of TERRA in MCF-10A were not significantly affected by either treatment (p > 0.05). (B) Total levels of TERRA in MCF-7 were not significantly affected by either treatment (p > 0.05). Data are ± SEM: for n = 3.