Skip to main content
. Author manuscript; available in PMC: 2014 Apr 11.
Published in final edited form as: Mol Cell. 2013 Mar 7;50(1):56–66. doi: 10.1016/j.molcel.2013.02.001

Figure 4. Overexpression of SRSF1 Leads to Senescence of Primary Fibroblasts through p53 Induction.

Figure 4

(A) BJ TT7-SRSF1 cells were induced with doxycycline for 7 d, fixed, stained with X-gal, and observed at 20× magnification (left panels). 200 cells were counted for each condition (right panel); n=6. Means +/− s.d. are shown; ***P = 0.0003.

(B) BJ TT7-SRSF1 cells were induced with doxycycline for 2, 4, or 7 d, incubated with 10 μM EdU, and observed at 20× magnification (top panels). 100 cells were counted for each condition (lower panel); n=6. Means +/− s.d. are shown; *P = 0.05, **P = 0.005, ***P = 0.0008.

(C) Wild-type or p53-null MEFs transduced with control or T7-SRSF1-expressing retroviruses were fixed and stained with X-gal (top panels). 200 cells were counted for each condition (lower panels); n=3. Means +/− s.d. are shown; *P = 0.05.

See also Figure S4.