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. Author manuscript; available in PMC: 2014 Apr 10.
Published in final edited form as: Neuron. 2013 Mar 21;78(1):138–151. doi: 10.1016/j.neuron.2013.01.030

Figure 2. Selective ablation of CGRPα primary afferents in dorsal spinal cord.

Figure 2

(AR) Immunostaining in lumbar spinal cord sections from adult CGRPα-DTR+/− mice. (A–L) hDTR marks CGRPα-expressing afferents. (M,P) PKCβII (red), (N,Q) PKCγ (red) and (O,R) CGRPα-GFP (green) mark populations of intrinsic dorsal spinal neurons. (M, N, P, Q) NeuN (blue). (O,R) IB4 (blue). Images were acquired by confocal microscopy. Scale bar in (L) is 100 μm and (Q,R) is 50 μm. All tissue collected 7 days after second saline/DTX injection. Images are representative of n=3 male mice per condition.