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. Author manuscript; available in PMC: 2014 Apr 10.
Published in final edited form as: Neuron. 2013 Mar 21;78(1):138–151. doi: 10.1016/j.neuron.2013.01.030

Figure 3. Ablation of CGRP-IR primary afferents in skin causes loss of peripheral nerve responses to noxious heat, while cold and mechanical responses are unaffected.

Figure 3

(A–F) Hindpaw glabrous skin from saline- and DTX-treated CGRPα-DTR+/− adult mice was stained with antibodies to (A,B) CGRP and (C,D) the pan-nerve fiber marker PGP9.5. (E,F) Merged images were stained with the nuclear marker DRAQ5 to facilitate visualization of skin cells. Images were acquired by confocal microscopy. Scale bar in (F) is 100 μm. CGRP-IR afferents were also ablated in hairy skin (Figure S2). Location of (G) laser heat responsive and (H) cold responsive spots in skin innervated by sural nerve; representative of n=3 animals per group. Filled circles=responsive spots. Open circles=non-responsive spots. (I, J) Representative multiunit electrophysiological recordings from the sural nerve before and after stimulation with the laser or cold solution. (K) Quantification of % heat and % cold responsive spots and number of laser-evoked action potentials in sural nerve as well as laser (heat), cold and mechanical threshold in isolated unmyelinated C-fibers. The number of recorded fibers is shown in parentheses. n=3 male mice/condition. Mean ± SEM. ***p<0.0005.