Table 1.

Quantification of heat, mechanical and cold behavioral assays.

Behavior	Male	Female
HEAT
Tail Immersion (46.5°C)	Latency to Flick (s)
Pre-Saline	16.9 ± 1.3	18.4 ± 1.4
Post-Saline	13.6 ± 1.5	16.7 ± 1.6
Pre-DTX	17.5 ± 1.4	17.6 ± 1.7
Post-DTX	30.0 ± 2.7**	29.4 ± 3.5*
Tail Immersion (49°C)	Latency to Flick (s)
Pre-Saline	5.3 ± 0.4	6.1 ± 0.3
Post-Saline	4.5 ± 0.4	5.8 ± 0.5
Pre-DTX	5.7 ± 0.4	5.8 ± 0.3
Post-DTX	13.6 ± 2.1**	17.3 ± 1.7***
Hot Plate (52°C)	Withdrawal Latency (s)
Pre-Saline	14.6 ± 1.6	17.2 ± 1.6
Post-Saline	15.6 ± 2.0	15.0 ± 1.3
Pre-DTX	14.6 ± 2.1	18.0 ± 1.5
Post-DTX	35.9 ± 3.5***	37.1 ± 3.3***
Capsaicin	Time Spent Licking (s)
Saline	42.3 ± 3.9	49.8 ± 1.9
DTX	18.5 ± 5.4**	24.7 ± 5.4**
Mechanical
Tail Clip	Latency to Bite Clip (s)
Saline	5.7 ± 1.5	3.7 ± 0.9
DTX	8.2 ± 2.0	4.1 ± 0.4
Cotton Swab	Paw Withdrawal Frequency (%)
Saline	34.3 ± 7.2	56 ± 7.5
DTX	51.3 ± 10.1	49 ± 5.5
COLD
Acetone	Time Spent Licking (s)
Pre-Saline	6.4 ± 1.0	7.1 ± 0.6
Post-Saline	4.9 ± 0.7	7.6 ± 1.0
Pre-DTX	6.0 ± 0.9	5.8 ± 0.5
Post-DTX	10.9 ± 2.0*	13.0 ± 1.5**
Tail Immersion (−10°C)	Latency to Flick (s)
Pre-Saline	48.2 ± 6.1	39.6 ± 3.1
Post-Saline	40.9 ± 5.3	37.8 ± 6.9
Pre-DTX	56.0 ± 6.4	40.1 ± 3.7
Post-DTX	26.3 ± 4.6**	28.6 ± 4.3*
Icilin	Number of Flinches
Saline	10.9 ± 1.6	15 ± 1.2
DTX	20.3 ± 1.7**	25 ± 3.4*
Cold Plantar Assay	Withdrawal Latency (s)
Pre-Saline	12.7 ± 0.8	10.4 ± 0.8
Post-Saline	12.4 ± 0.5	10.5 ± 0.6
Pre-DTX	11.3 ± 0.6	10.6 ± 0.6
Post-DTX	8.8 ± 0.4**	8.4 ± 0.4**

n=6–26 mice/group,

^*p<0.05,

^**p<0.005,

^***p<0.0005.