TABLE 1.

Primer sequences

Gene	Length of PCR product, bp	Forward	Reverse
GAPDH (NM_017008.3)	85	5′−TGACTCTACCCACGGCAAGT	5′−AGCATCACCCCATTTGATGT
Trim63 (MuRF1, NM_080903.1)	351	5′−GAGCAATTGCATCTCCATGCTGGT	5′−ACAATGCTCTTGATGAGCGGCTTG
Trim55 (MuRF2, NM_01012218.1)	245	5′−ACATAGGGAAACCTGGTGGAA	5′−TGTGTACACGCACCACACCTACA
Trim54 (MuRF3, NM_001013217.1)	185	5′−ACTTCACGGTGGGTTTCAAG	5′−TTGCCACAGAGGGTTAGAGG
Fbxo32 (MAFbx/atrogin1, NM_133521.1)	368	5′−TCTACACTGCGAACAGCAGCTGAA	5′−TCCAGGACAGAATGTGGCAGTGTT
Nppa (ANP, NM_012612.2)	166	5′−TGAAAAGCAAACTGAGGGCT	5′−GGATCTTTTGCGATCTGCTC

ANP Atrial natriuretic peptide; GAPDH Glyceraldehyde-3-phosphate dehydrogenase; MAFbx Muscle atrophy F-box; MuRF Muscle ring finger; PCR Polymerase chain reaction