Skip to main content
. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Antiviral Res. 2013 Feb 13;98(1):19–26. doi: 10.1016/j.antiviral.2013.02.001

Figure 1. Real-time IA-PCR specifically detects enveloped HBV DNA in G2.215 culture medium.

Figure 1

G2.215 cells were seeded in 96-well plates and incubated with lamivudine at different doses for 6 days. Cell culture medium was incubated with anti-HBs pre-immobilized on 96-well PCR plates at 4°C overnight. HBV DNA in captured virions was then amplified by real-time PCR. Medium from HepG2 cells and G2.215 cell medium incubated in wells coated with an anti-His antibody instead of anti-HBs were used as negative controls. A.) A dose-dependent increase in CT value was seen in medium from cells incubated with lamivudine. B.) The effect on CT levels was converted to reduction of HBV DNA by correlating to virus copy number using a standard curve of HBV genomic DNA. C). Serial CT dilution of HBV genomic DNA served as the standard curve for the real-time PCR assay.