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. 2013 Apr 15;201(2):309–323. doi: 10.1083/jcb.201301005

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© 2013 Romer et al.

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Figure 3. — Proliferative skeletal muscle precursor cells are present in the TZ. (A) P7 mice were injected with BrdU and 2 h later longitudinal sections of Cdo^+/− and Cdo^−/− esophagi were obtained and stained with antibodies to BrdU and Myf5 or α-SMA. The numbers of each panel (1, 2, 3) correspond to the equivalently numbered boxes/panels in Fig. 2. (B) Quantification of Myf5⁺ cells within the TZ, measured as a percentage of total DAPI⁺ cells in the ME. (C) Quantification of percentage of Myf5⁺ and α-SMA⁺ cells that are also positive for BrdU. Values for B and C are means ± SD, n = 4. (D) Longitudinal sections of P7 Cdo^+/+ esophagi were stained with antibodies to Pax7 and either Ki67, MyoD, or α-SMA, and with DAPI. Many Pax7⁺ cells coexpressed the proliferation marker Ki67, or the muscle determination marker MyoD (arrows in the respective panels), but Pax7⁺ cells did not express α-SMA. (E) Quantification of the percentage of Pax7⁺ cells in the TZ that are also either Ki67⁺ or MyoD⁺. Values are means ± SD, n = 4.