Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Apr 15;201(2):337–349. doi: 10.1083/jcb.201211155

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Avezov et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Fluorescence lifetime of ERroGFPiE is unaffected by physiological levels of unfolded protein stress. (A) A trace of time-dependent changes in ERroGFPiE fluorescence lifetime in live HEK 293T cells after exposure to 2 mM DTT, 5 µg/ml tunicamycin (TUN), 3 mM azetidine (AZT), or 2 µM thapsigargin (TG). Color-coded fluorescence lifetime images of cells 3 h into the exposure are shown in the bottom panel. Each data point represents the mean ± SD of fluorescence lifetime measured in ≥10 cells. (B) Fluorescent photomicrographs of cells as in A, immunostained for the ER stress–induced nuclear protein CHOP (left), GFP (middle), and a superposition of the two images with Hoechst nuclei staining (right). (C) Extended time course of color-coded fluorescence lifetime images of ERroGFPiE expressing live HEK 293T cells exposed to tunicamycin or azetidine. (D) A trace of time-dependent changes in ERroGFPiE fluorescence lifetime in untreated (green) and 3-h tunicamycin-treated HEK 293T cells (orange), before, during, and after brief exposure to DTT. (E) Extended time course of color-coded fluorescence lifetime images of ERroGFPiE expressing live HEK 293T cells exposed to 50 µg/ml of the protein synthesis inhibitors cycloheximide (CHX) or 10 µg/ml of puromycin (PUR). Shown are representative experiments reproduced twice.