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. 2013 Apr 15;201(2):337–349. doi: 10.1083/jcb.201211155

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© 2013 Avezov et al.

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Figure 6. — Failure of ER redox regulation at extreme unfolded protein stress. (A) Color-coded fluorescence lifetime images of ΔIre1α-deleted mouse cells and deleted cells rescued by an IRE1α transgene (IRE1⁺) after 4 h exposure to 5 µg/ml tunicamycin or 2 mM DTT. Values represent mean ± SD of fluorescence lifetime measured in ≥20 cells. (B) A trace of time-dependent changes in ERroGFPiE fluorescence lifetime in live COS7 cells that had been exposed to 5 µg/ml tunicamycin (TUN), 10 µM of the selective PERK kinase inhibitor GSK2606414 (PERKi), or both agents. Each data point represents the mean ± SD of fluorescence lifetime measured in ≥10 cells. (C) Color-coded fluorescence lifetime images along with histograms of lifetime frequency distribution of ERroGFPiE-expressing untreated COS7 cells and cells 3 h into the exposure to tunicamycin and PERK inhibitor. Shown are representative experiments reproduced twice.