. Author manuscript; available in PMC: 2014 May 1.

Published in final edited form as: Am J Surg Pathol. 2013 May;37(5):710–718. doi: 10.1097/PAS.0b013e3182772bba

Table 1.

Immunohistochemistry: Sources, Clones, Dilutions, and Pretreatment Conditions

Antibody	Source	Clone	Dilution	Pretreatment
EMA	Dako (Carpinteria, CA	E29	1:200	None
S100 protein	Dako	Polyclonal	1:3000	None
CK (pankeratin)	Dako	MNF-116	1:700	10 min protease digestion
CK (AE1/AE3)	Dako	AE1 + AE3	1:200	10 min protease digestion
CK (CAM 5.2)	Dako	CAM5.2	1:50	10 min protease digestion
GFAP	Dako	Polyclonal	1:15,000	Citrate buffer, pressure cooker
p63	Neomarkers, (Fremont, CA)	4A4	1:600	Citrate buffer, microwave
SMA	Sigma (St. Louis, MO)	1A4	1:20,000	None

Abbreviations: EMA, epithelial membrane antigen; CK, cytokeratin; SMA, smooth muscle actin; GFAP, glial fibrillary acidic protein