Table 1.
Immunohistochemistry: Sources, Clones, Dilutions, and Pretreatment Conditions
Antibody | Source | Clone | Dilution | Pretreatment |
---|---|---|---|---|
EMA | Dako (Carpinteria, CA | E29 | 1:200 | None |
S100 protein | Dako | Polyclonal | 1:3000 | None |
CK (pankeratin) | Dako | MNF-116 | 1:700 | 10 min protease digestion |
CK (AE1/AE3) | Dako | AE1 + AE3 | 1:200 | 10 min protease digestion |
CK (CAM 5.2) | Dako | CAM5.2 | 1:50 | 10 min protease digestion |
GFAP | Dako | Polyclonal | 1:15,000 | Citrate buffer, pressure cooker |
p63 | Neomarkers, (Fremont, CA) | 4A4 | 1:600 | Citrate buffer, microwave |
SMA | Sigma (St. Louis, MO) | 1A4 | 1:20,000 | None |
Abbreviations: EMA, epithelial membrane antigen; CK, cytokeratin; SMA, smooth muscle actin; GFAP, glial fibrillary acidic protein