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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Am J Surg Pathol. 2013 May;37(5):710–718. doi: 10.1097/PAS.0b013e3182772bba

Table 1.

Immunohistochemistry: Sources, Clones, Dilutions, and Pretreatment Conditions

Antibody Source Clone Dilution Pretreatment
EMA Dako (Carpinteria, CA E29 1:200 None
S100 protein Dako Polyclonal 1:3000 None
CK (pankeratin) Dako MNF-116 1:700 10 min protease digestion
CK (AE1/AE3) Dako AE1 + AE3 1:200 10 min protease digestion
CK (CAM 5.2) Dako CAM5.2 1:50 10 min protease digestion
GFAP Dako Polyclonal 1:15,000 Citrate buffer, pressure cooker
p63 Neomarkers, (Fremont, CA) 4A4 1:600 Citrate buffer, microwave
SMA Sigma (St. Louis, MO) 1A4 1:20,000 None

Abbreviations: EMA, epithelial membrane antigen; CK, cytokeratin; SMA, smooth muscle actin; GFAP, glial fibrillary acidic protein