Figure 2. Effect of temperature on the Km lactate of purified wood frog muscle LDH in the presence of 400 mM glucose, comparing LDH from control and dehydrated frogs.
Assays were at 7 °C, 22 °C and 31 °C, all in the presence of 400 mM glucose and at pH 8. Data are means ± SEM, n = 4 independent determinations. a – significantly different from the value for control LDH assayed at the same temperature using two-way ANOVA and Bonferroni Post Hoc test, p < 0.05; b – significantly different from the same animal state assayed at 22 °C (pH 8) using two-way ANOVA, p < 0.05.