Figure 3. Effect of temperature on the I₅₀ glucose for the reverse reaction of purified wood frog muscle LDH, assayed at 7 °C, 22 °C, and 30 °C.

Data are means ±SEM, n = 4 independent determinations. a – significantly different from the value for control LDH assayed at the same temperature using two-way ANOVA and Bonferroni Post Hoc test, p < 0.05; b – significantly different from the same animal state assayed at 22 °C (pH 8) using two-way ANOVA, p < 0.01.