Total RNA was isolated from cultured stromal vascular cells from 11 obese and 4 lean women. MCP1, IL-8, IL-6, TNF-α and leptin mRNA levels were measured by quantitative RT-PCR analysis. B. Expression of endotoxin sensitive genes is increased in isolated stromal vascular cells of obese pregnant women. Total RNA was isolated from cultured stromal vascular cells from 8 obese and 4 lean women. CD14, TLR4, and TRAM2 mRNA levels were measured by quantitative RT-PCR analysis. Data (mean ± SEM) were expressed as fold changes in obese vs. lean after normalization to β-actin. Statistical significance: * p< 0.05. Lean: open bars, obese: solid bars