Table 4. 16S diversity.
Bacterial 16S rRNA amplicon diversity analysis of two arid soil sites in Cuatro Cienegas, Coahuila, Mexico.
OTUs a | Singlets a | Doublets a | Chao1 | CI b | Shannon | CI b | Simpson | CI b | Ace | CI b | % coverage c | |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Dry lagoon | ||||||||||||
Before sterilization | 6586 | 4917 | 882 | 43226 | (39437–47450) | 7.31 | (7.27–7.34) | 0.007 | (0.006–0.007) | 99811 | (96592–103144) | 0.63 |
3 months | 4106 | 2760 | 587 | 20166 | (18253–22337) | 6.07 | (6.03–6.11) | 0.020 | (0.019–0.021) | 47720 | (45885–49636) | 0.78 |
6 months | 7518 | 5830 | 1093 | 42815 | (39583–46372) | 7.59 | (7.56–7.63) | 0.004 | (0.004–0.004) | 106755 | (103366–110262) | 0.61 |
9 months | 3552 | 2433 | 451 | 10097 | (9334–10961) | 7.17 | (7.15–7.21) | 0.002 | (0.002–0.003) | 18720 | (17980–19497) | 0.77 |
12 months | 6882 | 5114 | 808 | 60661 | (54582–67515) | 7.70 | (7.66–7.73) | 0.004 | (0.004–0.005) | 137126 | (133112–141267) | 0.58 |
Undisturbed soil | 8258 | 7157 | 1135 | 53773 | (49545–58434) | 8.16 | (8.13–8.19) | 0.001 | (0.001–0.001) | 130419 | (126816–134131) | 0.57 |
River | ||||||||||||
Before sterilization | 8268 | 6797 | 1085 | 53676 | (49534–58233) | 7.80 | (7.76–7.83) | 0.005 | (0.004–0.005) | 140680 | (136580–144910) | 0.56 |
3 months | 3785 | 2878 | 657 | 17395 | (15706–19323) | 6.04 | (6.00–6.08) | 0.013 | (0.013–0.014) | 32413 | (31077–33815) | 0.81 |
6 months | 9001 | 7289 | 1148 | 61455 | (56784–66583) | 8.24 | (8.21–8.26) | 0.001 | (0.001–0.002) | 161368 | (156754–166125) | 0.52 |
9 months | 6530 | 6607 | 1202 | 33236 | (30685–36056) | 7.35 | (7.32–7.38) | 0.003 | (0.003–0.003) | 94400 | (91445–97457) | 0.69 |
12 months | 6356 | 4728 | 734 | 27928 | (25926–30135) | 7.80 | (7.75–7.82) | 0.002 | (0.002–0.002) | 67224 | (65075–69452) | 0.62 |
Undisturbed soil | 8461 | 7019 | 1171 | 52934 | (48931–57332) | 8.23 | (8.20–8.25) | 0.001 | (0.001–0.001) | 125851 | (122223–129596) | 0.54 |
Notes.
All samples were normalized to 10,441 sequences (Table S3) for diversity comparison, values in parentheses represent the lower and upper 95% confidence interval associated with the Chao1 nonparametric estimator.
Values calculated based on 97% threshold.
Lower and upper 95% confident intervals associated with the diversity parameter.
Good’s coverage estimator.